› 5h4f

X-ray diffraction
2.05Å resolution

Structure of inorganic pyrophosphatase crystallised as a contaminant

Released:
DOI: 10.2210/pdb5h4f/pdb
PDB entry 5h4f coloured by chain, front view.
PDB entry 5h4f coloured by chain, side view.
PDB entry 5h4f coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structure determination of contaminant proteins using the MarathonMR procedure.
Hatti K, Biswas A, Chaudhary S, Dadireddy V, Sekar K, Srinivasan N, Murthy MRN
J Struct Biol 197 372-378 (2017)
PMID: 28167161

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 19.59 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: R32
Unit cell:
a: 108.098Å b: 108.098Å c: 155.607Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.211 0.251
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

findMySequence: a neural-network-based approach for identification of unknown proteins in X-ray crystallography and cryo-EM.
Chojnowski et al. (2022)
2 more

1 mention without citation

Identification, structure determination and analysis of Mycobacterium smegmatis acyl-carrier protein synthase (AcpS) crystallized serendipitously.
Bhatia et al. (2022)