› 5gja

X-ray diffraction
2.1Å resolution

Crystal structure of Arabidopsis thaliana ACO2 in complex with 2-PA

Released:
DOI: 10.2210/pdb5gja/pdb
PDB entry 5gja coloured by chain, front view.
PDB entry 5gja coloured by chain, side view.
PDB entry 5gja coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Pyrazinamide and derivatives block ethylene biosynthesis by inhibiting ACC oxidase.
Sun X, Li Y, He W, Ji C, Xia P, Wang Y, Du S, Li H, Raikhel N, Xiao J, Guo H
Nat Commun 8 15758 (2017)
PMID: 28604689

Function and Biology Details

Reaction catalysed: 
1-aminocyclopropane-1-carboxylate + ascorbate + O(2) = ethylene + cyanide + dehydroascorbate + CO(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-aminocyclopropane-1-carboxylate oxidase 2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 303 amino acids
Theoretical weight: 34.51 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q41931 (Residues: 1-303; Coverage: 95%)
Gene names: ACO2, At1g62380, EI305, F24O1.10
Sequence domains:
Structure domains: B-lactam Antibiotic, Isopenicillin N Synthase; Chain

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand 6PC 8 x 6PC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P1
Unit cell:
a: 69.455Å b: 95.343Å c: 95.892Å
α: 90.06° β: 89.48° γ: 89.99°
R-values:
R R work R free
0.171 0.171 0.218
Expression system: Escherichia coli

Quick links

Citations

6 review citations

1-Aminocyclopropane-1-Carboxylic Acid Oxidase (ACO): The Enzyme That Makes the Plant Hormone Ethylene.
Houben et al. (2019)
5 more

2 mentions without citation

Biological formation of ethylene.
Hausinger et al. (2023)
1 more