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S1 nuclease from Aspergillus oryzae in complex with phosphate and 2'-deoxycytidine

X-ray diffraction
1.75Å resolution
PDB entry 5fbd coloured by chain, front view.
PDB entry 5fbd coloured by chain, side view.
PDB entry 5fbd coloured by chain, top view.
Source organism: Aspergillus oryzae RIB40
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Nuclease S1
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Multimeric state: monomeric
Accessible surface area: 10750.74 Å2
Buried surface area: 1387.46 Å2
Dissociation area: 282.91 Å2
Dissociation energy (ΔGdiss): 3.79 kcal/mol
Dissociation entropy (TΔSdiss): 3.25 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-150145

Macromolecules

Nuclease S1
Chain: A
Length: 267 amino acids
Theoretical weight: 29.08 KDa
Source organism: Aspergillus oryzae RIB40
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P24021 (Residues: 21-287; Coverage: 100%)
Gene names: AO090001000075, nucS
Pfam: S1/P1 Nuclease
InterPro:
CATH: P1 Nuclease
Nuclease S1 in PDB entry 5fbd, assembly 1, front view.
Nuclease S1 in PDB entry 5fbd, assembly 1, side view.
Nuclease S1 in PDB entry 5fbd, assembly 1, top view.
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