› 5zql

X-ray diffraction
3.01Å resolution

crystal structure of human katanin AAA ATPase domain

Released:
DOI: 10.2210/pdb5zql/pdb
PDB entry 5zql coloured by chain, front view.
PDB entry 5zql coloured by chain, side view.
PDB entry 5zql coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and Molecular Basis for Katanin-Mediated Severing of Glutamylated Microtubules.
Shin SC, Im SK, Jang EH, Jin KS, Hur EM, Kim EE
Cell Rep 26 1357-1367.e5 (2019)
PMID: 30699360

Function and Biology Details

Reaction catalysed: 
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Katanin p60 ATPase-containing subunit A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 35.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75449 (Residues: 183-491; Coverage: 63%)
Gene name: KATNA1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P43
Unit cell:
a: 97.81Å b: 97.81Å c: 72.706Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.275 0.285
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The tubulin code and its role in controlling microtubule properties and functions.
Janke et al. (2020)
8 more