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Crystal structure of blue-shifted channelrhodopsin mutant (T198G/G202A)

X-ray diffraction
2.5Å resolution
PDB entry 4yzi coloured by chain, front view.
PDB entry 4yzi coloured by chain, side view.
PDB entry 4yzi coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Sensory opsin A, Sensory opsin B
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Multimeric state: homo dimer
Accessible surface area: 23664.19 Å2
Buried surface area: 10800.43 Å2
Dissociation area: 3,097.54 Å2
Dissociation energy (ΔGdiss): 20.56 kcal/mol
Dissociation entropy (TΔSdiss): 14.26 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-185251

Macromolecules

Sensory opsin A; Sensory opsin B
Chain: A
Length: 304 amino acids
Theoretical weight: 34.56 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8L435 (Residues: 39-245; Coverage: 30%)
  • Canonical: Q8RUT8 (Residues: 207-303; Coverage: 13%)
    • nullnull
Gene names: CHLRE_02g085257v5, CSOA, CSOB, acop2, cop4
Pfam: Bacteriorhodopsin-like protein
InterPro: Archaeal/bacterial/fungal rhodopsins
Sensory opsin A in PDB entry 4yzi, assembly 1, front view.
Sensory opsin A in PDB entry 4yzi, assembly 1, side view.
Sensory opsin A in PDB entry 4yzi, assembly 1, top view.
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