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E. coli YgjD(E12A)-YeaZ heterodimer in complex with ATP

X-ray diffraction
2.33Å resolution
PDB entry 4wq4 coloured by chain, front view.
PDB entry 4wq4 coloured by chain, side view.
PDB entry 4wq4 coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: tRNA N6-adenosine threonylcarbamoyltransferase and tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
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Multimeric state: hetero dimer
Accessible surface area: 21960.88 Å2
Buried surface area: 4608.95 Å2
Dissociation area: 1,432.35 Å2
Dissociation energy (ΔGdiss): 15.39 kcal/mol
Dissociation entropy (TΔSdiss): 12.89 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-138800
Assembly 2
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Assembly Name: tRNA N6-adenosine threonylcarbamoyltransferase and tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
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Multimeric state: hetero dimer
Accessible surface area: 21938.81 Å2
Buried surface area: 4323.25 Å2
Dissociation area: 1,492.46 Å2
Dissociation energy (ΔGdiss): 10.25 kcal/mol
Dissociation entropy (TΔSdiss): 12.89 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-138800

Macromolecules

tRNA N6-adenosine threonylcarbamoyltransferase
Chains: A, B
Length: 343 amino acids
Theoretical weight: 36.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Best match: P05852 (Residues: 1-337)
Gene names: JW3036, b3064, gcp, tsaD, ygjD
tRNA N6-adenosine threonylcarbamoyltransferase in PDB entry 4wq4, assembly 1, front view.
tRNA N6-adenosine threonylcarbamoyltransferase in PDB entry 4wq4, assembly 1, side view.
tRNA N6-adenosine threonylcarbamoyltransferase in PDB entry 4wq4, assembly 1, top view.
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tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Chains: C, D
Length: 237 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Best match: P76256 (Residues: 1-231)
Gene names: JW1796, b1807, tsaB, yeaZ
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB in PDB entry 4wq4, assembly 1, front view.
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB in PDB entry 4wq4, assembly 1, side view.
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB in PDB entry 4wq4, assembly 1, top view.
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