› 4uqx

X-ray diffraction
1.2Å resolution

Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes

Released:
DOI: 10.2210/pdb4uqx/pdb
PDB entry 4uqx coloured by chain, front view.
PDB entry 4uqx coloured by chain, side view.
PDB entry 4uqx coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Coevolution of the ATPase ClpV, the sheath proteins TssB and TssC, and the accessory protein TagJ/HsiE1 distinguishes type VI secretion classes.
Förster A, Planamente S, Manoli E, Lossi NS, Freemont PS, Filloux A
OpenAccess logo J Biol Chem 289 33032-43 (2014)
PMID: 25305017

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191492 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type VI secretion system accessory component TagJ Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 28.67 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q9I746 (Residues: 21-281; Coverage: 93%)
Gene names: PA0086, tagJ
Sequence domains:
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

2 bound ligands:
Ligand MPD 2 x MPD
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 56.76Å b: 45.87Å c: 59.47Å
α: 90° β: 109.98° γ: 90°
R-values:
R R work R free
0.151 0.151 0.158
Expression system: Escherichia coli B

Quick links

Citations

10 review citations

Aim, Load, Fire: The Type VI Secretion System, a Bacterial Nanoweapon.
Cianfanelli et al. (2016)
9 more

1 mention without citation

Coevolution of the ATPase ClpV, the sheath proteins TssB and TssC, and the accessory protein TagJ/HsiE1 distinguishes type VI secretion classes.
Förster et al. (2014)