› › Structure analysis

Berberine bridge enzyme G164A variant, a reticuline dehydrogenase

X-ray diffraction
2.2Å resolution
PDB entry 4pzf coloured by chain, front view.
PDB entry 4pzf coloured by chain, side view.
PDB entry 4pzf coloured by chain, top view.
Source organism: Eschscholzia californica
Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents: 1 distinct polypeptide molecule

Quick links

Assemblies

Assembly 1
Download    3D Visualisation
Assembly Name: Reticuline oxidase
hide full assembly name
Multimeric state: monomeric
Accessible surface area: 19343.43 Å2
Buried surface area: 2099.51 Å2
Dissociation area: 152.48 Å2
Dissociation energy (ΔGdiss): -6.83 kcal/mol
Dissociation entropy (TΔSdiss): 2.97 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-151854
Assembly 2
Download    3D Visualisation
Assembly Name: Reticuline oxidase
hide full assembly name
Multimeric state: monomeric
Accessible surface area: 19583.43 Å2
Buried surface area: 2186.01 Å2
Dissociation area: 65.07 Å2
Dissociation energy (ΔGdiss): 7.07 kcal/mol
Dissociation entropy (TΔSdiss): 0.59 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-151854
Assembly 3 (preferred)
Download    3D Visualisation
Assembly Name: Reticuline oxidase
hide full assembly name
Multimeric state: monomeric
Accessible surface area: 20146.96 Å2
Buried surface area: 2906.47 Å2
Dissociation area: 274.27 Å2
Dissociation energy (ΔGdiss): 1.36 kcal/mol
Dissociation entropy (TΔSdiss): 5.33 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-151854
Assembly 4
Download    3D Visualisation
Assembly Name: Reticuline oxidase
hide full assembly name
Multimeric state: monomeric
Accessible surface area: 19407.83 Å2
Buried surface area: 2971.95 Å2
Dissociation area: 258.18 Å2
Dissociation energy (ΔGdiss): -1.98 kcal/mol
Dissociation entropy (TΔSdiss): 4.62 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-151854
Assembly 5
Download    3D Visualisation
Assembly Name: Reticuline oxidase
hide full assembly name
Multimeric state: homo dimer
Accessible surface area: 37565.89 Å2
Buried surface area: 6930.49 Å2
Dissociation area: 969.08 Å2
Dissociation energy (ΔGdiss): 3.05 kcal/mol
Dissociation entropy (TΔSdiss): 14.47 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-151855
Assembly 6
Download    3D Visualisation
Assembly Name: Reticuline oxidase
hide full assembly name
Multimeric state: homo dimer
Accessible surface area: 37151.39 Å2
Buried surface area: 6997.83 Å2
Dissociation area: 942 Å2
Dissociation energy (ΔGdiss): 2.59 kcal/mol
Dissociation entropy (TΔSdiss): 14.47 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-151855

Macromolecules

Reticuline oxidase
Chains: A, B, C, D
Length: 538 amino acids
Theoretical weight: 60.06 KDa
Source organism: Eschscholzia californica
Expression system: Komagataella pastoris
UniProt:
  • Best match: P30986 (Residues: 1-538)
Gene name: BBE1
Reticuline oxidase in PDB entry 4pzf, assembly 3, front view.
Reticuline oxidase in PDB entry 4pzf, assembly 3, side view.
Reticuline oxidase in PDB entry 4pzf, assembly 3, top view.
Molecule details ›

Search similar proteins

Quick links