› 4k02

X-ray diffraction
1.9Å resolution

Crystal structure of AtDHNAT1, a 1,4-dihydroxy-2-naphthoyl-CoA thioesterase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb4k02/pdb
PDB entry 4k02 coloured by chain, front view.
PDB entry 4k02 coloured by chain, side view.
PDB entry 4k02 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Functional convergence of structurally distinct thioesterases from cyanobacteria and plants involved in phylloquinone biosynthesis.
Furt F, Allen WJ, Widhalm JR, Madzelan P, Rizzo RC, Basset G, Wilson MA
Acta Crystallogr D Biol Crystallogr 69 1876-88 (2013)
PMID: 24100308

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,4-dihydroxy-2-naphthoyl-CoA thioesterase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 17.02 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SX65 (Residues: 1-156; Coverage: 100%)
Gene names: At1g48320, DHNAT1, F11A17.13
Sequence domains: Thioesterase superfamily
Structure domains: Hotdog Thioesterase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3121
Unit cell:
a: 99.529Å b: 99.529Å c: 61.257Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Biosynthesis and applications of prenylquinones.
Kawamukai M. (2018)
1 more

2 mentions without citation

Functional convergence of structurally distinct thioesterases from cyanobacteria and plants involved in phylloquinone biosynthesis.
Furt et al. (2013)
1 more