› 4jbh

X-ray diffraction
2.2Å resolution

2.2A resolution structure of cobalt and zinc bound thermostable alcohol dehydrogenase from Pyrobaculum aerophilum

Released:
DOI: 10.2210/pdb4jbh/pdb
PDB entry 4jbh coloured by chain, front view.
PDB entry 4jbh coloured by chain, side view.
PDB entry 4jbh coloured by chain, top view.
Source organism: Pyrobaculum aerophilum str. IM2
Primary publication:
Physicochemical characterization of a thermostable alcohol dehydrogenase from Pyrobaculum aerophilum.
Vitale A, Thorne N, Lovell S, Battaile KP, Hu X, Shen M, D'Auria S, Auld DS
OpenAccess logo PLoS One 8 e63828 (2013)
PMID: 23755111

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl reductase (ER) domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 370 amino acids
Theoretical weight: 38.69 KDa
Source organism: Pyrobaculum aerophilum str. IM2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZUP0 (Residues: 1-331; Coverage: 100%)
Gene name: PAE2687
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand CO 4 x CO
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 68.402Å b: 125.336Å c: 82.943Å
α: 90° β: 107.12° γ: 90°
R-values:
R R work R free
0.153 0.151 0.195
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Decarboxylation of pyruvate to acetaldehyde for ethanol production by hyperthermophiles.
Eram et al. (2013)
1 more

1 mention without citation

Physicochemical characterization of a thermostable alcohol dehydrogenase from Pyrobaculum aerophilum.
Vitale et al. (2013)