› 4zz4

Neutron Diffraction
1.8Å resolution

Neutron crystal structure of ribonuclease A determined by the real space D/H contrast method

Released:
DOI: 10.2210/pdb4zz4/pdb
PDB entry 4zz4 coloured by chain, front view.
PDB entry 4zz4 coloured by chain, side view.
PDB entry 4zz4 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A technique for determining the deuterium/hydrogen contrast map in neutron macromolecular crystallography.
Chatake T, Fujiwara S
Acta Crystallogr D Struct Biol 72 71-82 (2016)
PMID: 26894536

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand IPA 1 x IPA
Ligand DOD 107 x DOD
No modified residues

Experiments and Validation Details

Spacegroup: P21
Unit cell:
a: 30.34Å b: 38.369Å c: 53.188Å α: 90° β: 105.75° γ: 90°
R-values:
R R work R free 0.199 0.197 0.22

Quick links

Citations

1 review citation

The Advances and Limitations of the Determination and Applications of Water Structure in Molecular Engineering.
Zsidó et al. (2023)
1 other article

2 mentions without citation

Evaluation of models determined by neutron diffraction and proposed improvements to their validation and deposition.
Liebschner et al. (2018)
1 more