Pathways & interactions
Ribonuclease A, active site (IPR023411)
Short name: RNaseA_AS
Pancreatic ribonucleases (RNaseA) are pyrimidine-specific endonucleases found in high quantity in the pancreas of certain mammals and of some reptiles [PMID: 3940901]. Specifically, the enzymes are involved in endonucleolytic cleavage of 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates. Ribonuclease can unwind the DNA helix by complexing with single-stranded DNA; the complex arises by an extended multi-site cation-anion interaction between lysine and arginine residues of the enzyme and phosphate groups of the nucleotides. Other proteins belonging to the pancreatic RNAse family include: bovine seminal vesicle and brain ribonucleases; kidney non-secretory ribonucleases [PMID: 2734298]; liver-type ribonucleases [PMID: 2611266]; angiogenin, which induces vascularisation of normal and malignant tissues; eosinophil cationic protein [PMID: 2473157], a cytotoxin and helminthotoxin with ribonuclease activity; and frog liver ribonuclease and frog sialic acid-binding lectin. The sequence of pancreatic RNases contains four conserved disulphide bonds and three amino acid residues involved in the catalytic activity.
Pancreatic ribonucleases (EC:126.96.36.199) are pyrimidine-specific endonucleases present in high quantity in the pancreas of a number of mammalian taxa and of a few reptiles [PMID: 3074337, PMID: 3940901]. As shown in the following schematic representation of the sequence of pancreatic RNases there are four conserved disulphide bonds and three amino acid residues involved in the catalytic activity.
+---------------------------+ | +------------------|------+ | | | | xxxxx#xxxxxxCxxxxxxC#xxxxxxxCxxCxxxCxxxxxCxxxxxCxxxxxxCxxx#xxx | **** | | | | +---+ | +----------------------------+ 'C': conserved cysteine involved in a disulphide bond. '#': active site residue. '*': position of the pattern.
A number of other proteins belongs to the pancreatic RNAse family and these are listed below.
- Bovine seminal vesicle and bovine brain ribonucleases.
- The kidney non-secretory ribonucleases (also known as eosinophil-derived neurotoxin (EDN) [PMID: 2734298]).
- Liver-type ribonucleases [PMID: 2611266].
- Angiogenin, which induces vascularization of normal and malignant tissues. It abolishes protein synthesis by specifically hydrolyzing cellular tRNAs.
- Eosinophil cationic protein (ECP) [PMID: 2473157], a cytotoxin and helminthotoxin with ribonuclease activity.
- Frog liver ribonuclease and frog sialic acid-binding lectin [PMID: 2710786].
The signature pattern for these proteins includes five conserved residues: a cysteine involved in a disulphide bond, a lysine involved in the catalytic activity and three other residues important for substrate binding.
- PS00127 (RNASE_PANCREATIC)