X-ray diffraction
1.98Å resolution

Ergothioneine-biosynthetic sulfoxide synthase EgtB in complex with N,N-dimethyl-histidine and gamma-glutamyl-cysteine


Function and Biology Details

Reaction catalysed:
Hercynine + gamma-L-glutamyl-L-cysteine + O(2) = gamma-L-glutamyl-S-hercyn-2-yl-L-cysteine S-oxide + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Hercynine oxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 447 amino acids
Theoretical weight: 49.56 KDa
Source organism: Mycolicibacterium thermoresistibile ATCC 19527
Expression system: Escherichia coli BL21(DE3)
  • Canonical: G7CFI3 (Residues: 3-446; Coverage: 100%)
Gene names: KEK_08772, egtB
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 135.292Å b: 135.292Å c: 141.293Å
α: 90° β: 90° γ: 90°
R R work R free
0.152 0.15 0.173
Expression system: Escherichia coli BL21(DE3)