› 4oy7

X-ray diffraction
1.5Å resolution

Structure of cellulose active LPMO CelS2 (ScLPMO10C) in complex with Copper.

Released:
DOI: 10.2210/pdb4oy7/pdb
PDB entry 4oy7 coloured by chain, front view.
PDB entry 4oy7 coloured by chain, side view.
PDB entry 4oy7 coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases.
Forsberg Z, Mackenzie AK, Sørlie M, Røhr ÅK, Helland R, Arvai AS, Vaaje-Kolstad G, Eijsink VG
Proc Natl Acad Sci U S A 111 8446-51 (2014)
PMID: 24912171

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193185 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CBM2 domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 196 amino acids
Theoretical weight: 21 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RJY2 (Residues: 35-230; Coverage: 54%)
Gene names: SCG11A.19, SCO1188, celS2
Sequence domains: Lytic polysaccharide mono-oxygenase, cellulose-degrading
Structure domains: chitin-binding protein cbp21

Ligands and Environments

2 bound ligands:
Ligand CU 8 x CU
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 83.858Å b: 122.856Å c: 156Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Cellulose degradation by polysaccharide monooxygenases.
Beeson et al. (2015)
19 more

19 mentions without citation

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass.
Bissaro et al. (2018)
18 more