› 4niq

X-ray diffraction
2.3Å resolution

Crystal Structure of Vps4 MIT-Vfa1 MIM2

Released:
DOI: 10.2210/pdb4niq/pdb
PDB entry 4niq coloured by chain, front view.
PDB entry 4niq coloured by chain, side view.
PDB entry 4niq coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Vfa1 binds to the N-terminal microtubule-interacting and trafficking (MIT) domain of Vps4 and stimulates its ATPase activity.
Vild CJ, Xu Z
J Biol Chem 289 10378-10386 (2014)
PMID: 24567329

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154018 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vacuolar protein sorting-associated protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 83 amino acids
Theoretical weight: 9.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P52917 (Residues: 1-82; Coverage: 19%)
Gene names: CSC1, DID6, END13, GRD13, P9705.10, VPL4, VPS4, VPT10, YPR173C
Sequence domains: MIT (microtubule interacting and transport) domain
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit
VPS4-associated protein 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 23 amino acids
Theoretical weight: 2.69 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40080 (Residues: 182-203; Coverage: 11%)
Gene names: SYGP-ORF44, VFA1, YER128W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 38.476Å b: 56.711Å c: 84.233Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.257
Expression system: Escherichia coli

Quick links

Citations

2 review citations

ESCRT-III and Vps4: a dynamic multipurpose tool for membrane budding and scission.
Alonso Y Adell et al. (2016)
1 more

1 mention without citation

Vfa1 binds to the N-terminal microtubule-interacting and trafficking (MIT) domain of Vps4 and stimulates its ATPase activity.
Vild et al. (2014)