› 4ld1

X-ray diffraction
1.44Å resolution

Structural analysis of the microcephaly protein CPAP G-box domain suggests a role in centriole elongation.

Released:
DOI: 10.2210/pdb4ld1/pdb
PDB entry 4ld1 coloured by chain, front view.
PDB entry 4ld1 coloured by chain, side view.
PDB entry 4ld1 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structural analysis of the G-box domain of the microcephaly protein CPAP suggests a role in centriole architecture.
Hatzopoulos GN, Erat MC, Cutts E, Rogala KB, Slater LM, Stansfeld PJ, Vakonakis I
Structure 21 2069-77 (2013)
PMID: 24076405

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Centromere protein J C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.86 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: E7FCY1 (Residues: 942-1121; Coverage: 16%)
Gene name: cenpj
Sequence domains: T-complex protein 10 C-terminus
Structure domains: lipopolysaccharide transport protein A fold

Ligands and Environments

4 bound ligands:
Ligand NO3 2 x NO3
Ligand PGE 1 x PGE
Ligand PG4 1 x PG4
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 41.632Å b: 50.254Å c: 60.345Å
α: 90° β: 108.56° γ: 90°
R-values:
R R work R free
0.155 0.154 0.18
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Centrosome function and assembly in animal cells.
Conduit et al. (2015)
18 more

3 mentions without citation

The centriolar protein CPAP G-box: an amyloid fibril in a single domain.
Cutts et al. (2015)
2 more