4jhd

X-ray diffraction
2.91Å resolution

Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-145605 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin-5C Chains: A, D
Molecule details ›
Chains: A, D
Length: 384 amino acids
Theoretical weight: 42.99 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10987 (Residues: 1-376; Coverage: 100%)
Gene names: Act5C, CG4027
Sequence domains: Actin
Structure domains:
Actin-5C Chains: B, E
Molecule details ›
Chains: B, E
Length: 384 amino acids
Theoretical weight: 42.93 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10987 (Residues: 1-376; Coverage: 100%)
Gene names: Act5C, CG4027
Sequence domains: Actin
Structure domains:
Protein cordon-bleu Chains: C, F
Molecule details ›
Chains: C, F
Length: 171 amino acids
Theoretical weight: 18.14 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NBX1 (Residues: 1176-1337; Coverage: 12%)
Gene names: Cobl, Kiaa0633
Sequence domains: WH2 motif

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 53.45Å b: 99.8Å c: 118.27Å
α: 65.41° β: 90.03° γ: 77.77°
R-values:
R R work R free
0.203 0.2 0.255
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli