› 3wic

X-ray diffraction
2.6Å resolution

Structure of a substrate/cofactor-unbound glucose dehydrogenase

Released:
DOI: 10.2210/pdb3wic/pdb
PDB entry 3wic coloured by chain, front view.
PDB entry 3wic coloured by chain, side view.
PDB entry 3wic coloured by chain, top view.
Source organism: Thermoplasma volcanium GSS1
Primary publication:
Structural insight into glucose dehydrogenase from the thermoacidophilic archaeon Thermoplasma volcanium.
Kanoh Y, Uehara S, Iwata H, Yoneda K, Ohshima T, Sakuraba H
Acta Crystallogr D Biol Crystallogr 70 1271-80 (2014)
PMID: 24816096

Function and Biology Details

Reaction catalysed: 
D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188869 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose 1-dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 361 amino acids
Theoretical weight: 40.3 KDa
Source organism: Thermoplasma volcanium GSS1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q979W2 (Residues: 1-361; Coverage: 100%)
Gene names: TVG1073292, gdh
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand PGO 6 x PGO
Ligand 1PE 8 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 79.088Å b: 122.175Å c: 87.428Å
α: 90° β: 109.92° γ: 90°
R-values:
R R work R free
0.208 0.205 0.256
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Characterization of a thermostable glucose dehydrogenase with strict substrate specificity from a hyperthermophilic archaeon Thermoproteus sp. GDH-1.
Aiba et al. (2015)