› 3uve

X-ray diffraction
1.55Å resolution

Crystal structure of Carveol dehydrogenase ((+)-trans-carveol dehydrogenase) from Mycobacterium avium

Released:
DOI: 10.2210/pdb3uve/pdb
PDB entry 3uve coloured by chain, front view.
PDB entry 3uve coloured by chain, side view.
PDB entry 3uve coloured by chain, top view.
Source organism: Mycobacterium avium 104
Primary publication:
Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ
Tuberculosis (Edinb) 95 142-8 (2015)
PMID: 25613812

Function and Biology Details

Reaction catalysed: 
(+)-trans-carveol + NAD(+) = (+)-(S)-carvone + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carveol dehydrogenase ((+)-trans-carveol dehydrogenase) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 286 amino acids
Theoretical weight: 30.31 KDa
Source organism: Mycobacterium avium 104
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2ZTN5 (Residues: 1-282; Coverage: 100%)
Gene name: MAV_0896
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand PG4 4 x PG4
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 97.61Å b: 59.44Å c: 108.65Å
α: 90° β: 90.1° γ: 90°
R-values:
R R work R free
0.146 0.146 0.16
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Pleiotropic Functions of Tumor Suppressor WWOX in Normal and Cancer Cells.
Abu-Remaileh et al. (2015)
5 more

3 mentions without citation

Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors.
Haft et al. (2017)
2 more