› 3u5y

X-ray diffraction
2.3Å resolution

Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity

Released:
DOI: 10.2210/pdb3u5y/pdb
PDB entry 3u5y coloured by chain, front view.
PDB entry 3u5y coloured by chain, side view.
PDB entry 3u5y coloured by chain, top view.
Source organism: Rauvolfia serpentina
Primary publication:
Structures of alkaloid biosynthetic glucosidases decode substrate specificity.
Xia L, Ruppert M, Wang M, Panjikar S, Lin H, Rajendran C, Barleben L, Stöckigt J
ACS Chem Biol 7 226-34 (2012)
PMID: 22004291

Function and Biology Details

Reactions catalysed: 
UDP-glucose + vomilenine = UDP + raucaffricine
Raucaffricine + H(2)O = D-glucose + vomilenine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo octamer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193498 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Raucaffricine-O-beta-D-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 57.83 KDa
Source organism: Rauvolfia serpentina
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SPP9 (Residues: 1-513; Coverage: 95%)
Gene names: RG, VGT
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand SCG 2 x SCG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I222
Unit cell:
a: 104.927Å b: 129.203Å c: 216.798Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.231
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Gates of enzymes.
Gora et al. (2013)
2 more

1 mention without citation

Functional Characterization of Genes Coding for Novel β-D-Glucosidases Involved in the Initial Step of Secoiridoid Glucosides Catabolism in Centaurium erythraea Rafn.
Božunović et al. (2022)