› 3tpf

X-ray diffraction
2.7Å resolution

Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168

Released:
DOI: 10.2210/pdb3tpf/pdb
PDB entry 3tpf coloured by chain, front view.
PDB entry 3tpf coloured by chain, side view.
PDB entry 3tpf coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Primary publication:
Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 Å resolution.
Shabalin IG, Porebski PJ, Cooper DR, Grabowski M, Onopriyenko O, Grimshaw S, Savchenko A, Chruszcz M, Minor W
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 1018-24 (2012)
PMID: 22949186

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ornithine carbamoyltransferase, anabolic Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 307 amino acids
Theoretical weight: 35.69 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9PNU6 (Residues: 1-306; Coverage: 100%)
Gene names: Cj0994c, argF
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

1 bound ligand:
Ligand PEG 3 x PEG
1 modified residue:
Ligand MSE 78 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 157.163Å b: 87.398Å c: 141.561Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.24
Expression system: Escherichia coli

Quick links

Citations

1 review citation

From Genome to Structure and Back Again: A Family Portrait of the Transcarbamylases.
Shi et al. (2015)
2 other articles

2 mentions without citation

New insight into the transcarbamylase family: the structure of putrescine transcarbamylase, a key catalyst for fermentative utilization of agmatine.
Polo et al. (2012)
1 more