› 3hl3

X-ray diffraction
2.76Å resolution

2.76 Angstrom Crystal Structure of a Putative Glucose-1-Phosphate Thymidylyltransferase from Bacillus anthracis in Complex with a Sucrose.

Released:
DOI: 10.2210/pdb3hl3/pdb
PDB entry 3hl3 coloured by chain, front view.
PDB entry 3hl3 coloured by chain, side view.
PDB entry 3hl3 coloured by chain, top view.
Source organism: Bacillus anthracis
Entry authors: Minasov G, Shuvalova L, Halavaty A, Dubrovska I, Winsor J, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
glucose-1-phosphate thymidylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 269 amino acids
Theoretical weight: 30.28 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L8PCC3 (Residues: 1-245; Coverage: 100%)
Gene name: GBAA_1228
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P622
Unit cell:
a: 134.425Å b: 134.425Å c: 85.274Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.206
Expression system: Escherichia coli

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