› 3bzw

X-ray diffraction
1.87Å resolution

Crystal structure of a putative lipase from Bacteroides thetaiotaomicron

Released:
DOI: 10.2210/pdb3bzw/pdb
PDB entry 3bzw coloured by chain, front view.
PDB entry 3bzw coloured by chain, side view.
PDB entry 3bzw coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Entry authors: Palani K, Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SGNH hydrolase-type esterase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 274 amino acids
Theoretical weight: 31.84 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8A3J3 (Residues: 23-285; Coverage: 100%)
Gene name: BT_2961
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase

Ligands and Environments

2 bound ligands:
Ligand ACT 6 x ACT
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 54 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 104.225Å b: 107.548Å c: 144.919Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.215
Expression system: Escherichia coli BL21(DE3)

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