› 3el2

X-ray diffraction
2.5Å resolution

Crystal Structure of Monomeric Actin Bound to Ca-ATP

Released:
DOI: 10.2210/pdb3el2/pdb
PDB entry 3el2 coloured by chain, front view.
PDB entry 3el2 coloured by chain, side view.
PDB entry 3el2 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structures of monomeric actin bound to cytochalasin D.
Nair UB, Joel PB, Wan Q, Lowey S, Rould MA, Trybus KM
J Mol Biol 384 848-64 (2008)
PMID: 18938176

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-5C Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.83 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10987 (Residues: 2-376; Coverage: 100%)
Gene names: Act5C, CG4027
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 1 x ATP
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 206.634Å b: 54.023Å c: 36.972Å
α: 90° β: 98.16° γ: 90°
R-values:
R R work R free
0.231 0.203 0.262
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Control of actin filament treadmilling in cell motility.
Bugyi et al. (2010)
3 more

7 mentions without citation

Actin-Interacting Amphidinolides: Syntheses and Mechanisms of Action of Amphidinolides X, J, and K.
Costa AM. (2023)
6 more