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NMR structure of the first qRRM of hnRNP F in complex with AGGGAU G-tract RNA

Solution NMR
PDB entry 2kfy coloured by chain, ensemble of 20 models, front view.
PDB entry 2kfy coloured by chain, ensemble of 20 models, side view.
PDB entry 2kfy coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Heterogeneous nuclear ribonucleoprotein F, N-terminally processed and RNA
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Multimeric state: hetero dimer
Accessible surface area: 7167.77 Å2
Buried surface area: 1392.49 Å2
Dissociation area: 696.25 Å2
Dissociation energy (ΔGdiss): -4.46 kcal/mol
Dissociation entropy (TΔSdiss): 8.29 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-156581

Macromolecules

Heterogeneous nuclear ribonucleoprotein F, N-terminally processed
Chain: A
Length: 136 amino acids
Theoretical weight: 15.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P52597 (Residues: 1-102; Coverage: 25%)
Gene names: HNRNPF, HNRPF
InterPro:
CATH: Alpha-Beta Plaits
Heterogeneous nuclear ribonucleoprotein F, N-terminally processed in PDB entry 2kfy, assembly 1, front view.
Heterogeneous nuclear ribonucleoprotein F, N-terminally processed in PDB entry 2kfy, assembly 1, side view.
Heterogeneous nuclear ribonucleoprotein F, N-terminally processed in PDB entry 2kfy, assembly 1, top view.
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RNA chain B
Name: 5'-R(*AP*GP*GP*GP*AP*U)-3'
Representative chains: B
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 6 nucleotides
Theoretical weight: 1.96 KDa
5'-R(*AP*GP*GP*GP*AP*U)-3' in PDB entry 2kfy, assembly 1, front view.
5'-R(*AP*GP*GP*GP*AP*U)-3' in PDB entry 2kfy, assembly 1, side view.
5'-R(*AP*GP*GP*GP*AP*U)-3' in PDB entry 2kfy, assembly 1, top view.
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