2i3s

X-ray diffraction
1.9Å resolution

Bub3 complex with Bub1 GLEBS motif

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural analysis of Bub3 interactions in the mitotic spindle checkpoint.
Proc. Natl. Acad. Sci. U.S.A. 104 1201-6 (2007)
PMID: 17227844

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell cycle arrest protein BUB3 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 349 amino acids
Theoretical weight: 39.55 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P26449 (Residues: 1-341; Coverage: 100%)
Gene names: BUB3, OR26.16, YOR026W
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Checkpoint serine/threonine-protein kinase BUB1 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 36 amino acids
Theoretical weight: 4.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P41695 (Residues: 315-350; Coverage: 4%)
Gene names: BUB1, G7542, YGR188C

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 50.656Å b: 79.181Å c: 142.582Å
α: 90° β: 98.34° γ: 90°
R-values:
R R work R free
0.221 0.219 0.262
Expression systems:
  • Escherichia coli
  • Not provided