› 1tuw

X-ray diffraction
1.9Å resolution

Structural and Functional Analysis of Tetracenomycin F2 Cyclase from Streptomyces glaucescens: A Type-II Polyketide Cyclase

Released:
DOI: 10.2210/pdb1tuw/pdb
PDB entry 1tuw coloured by chain, front view.
PDB entry 1tuw coloured by chain, side view.
PDB entry 1tuw coloured by chain, top view.
Source organism: Streptomyces glaucescens
Primary publication:
Structural and functional analysis of tetracenomycin F2 cyclase from Streptomyces glaucescens. A type II polyketide cyclase.
Thompson TB, Katayama K, Watanabe K, Hutchinson CR, Rayment I
J Biol Chem 279 37956-63 (2004)
PMID: 15231835

Function and Biology Details

Reaction catalysed: 
Tetracenomycin F2 = tetracenomycin F1 + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracenomycin F2 cyclase Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.88 KDa
Source organism: Streptomyces glaucescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39890 (Residues: 1-109; Coverage: 100%)
Gene name: tcmI
Sequence domains: Polyketide synthesis cyclase
Structure domains: Dimeric alpha+beta barrel.

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6522
Unit cell:
a: 46.7Å b: 46.7Å c: 188.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.245
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Total biosynthesis: in vitro reconstitution of polyketide and nonribosomal peptide pathways.
Sattely et al. (2008)
5 more

2 mentions without citation

Identification of olivetolic acid cyclase from Cannabis sativa reveals a unique catalytic route to plant polyketides.
Gagne et al. (2012)
1 more