Solution NMR

The Mad2 Spindle Checkpoint Protein Undergoes Similar Major Conformational Changes upon Binding to Either Mad1 or Cdc20


Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2A Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 22.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q13257 (Residues: 11-205; Coverage: 95%)
Gene names: MAD2, MAD2L1
Sequence domains: HORMA domain
Structure domains: HORMA domain
Mad2-binding peptide Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 76%
Refinement method: torsion angle dynamics
Expression systems:
  • Escherichia coli
  • Not provided