› 1yxw

X-ray diffraction
2.2Å resolution

A common binding site for disialyllactose and a tri-peptide in the C-fragment of tetanus neurotoxin

Released:
DOI: 10.2210/pdb1yxw/pdb
PDB 1yxw coloured by chain and viewed from the front.
PDB 1yxw coloured by chain and viewed from the side.
PDB 1yxw coloured by chain and viewed from the top.
Source organism: Clostridium tetani
Primary publication:
Common binding site for disialyllactose and tri-peptide in C-fragment of tetanus neurotoxin.
Jayaraman S, Eswaramoorthy S, Kumaran D, Swaminathan S
Proteins 61 288-95 (2005)
PMID: 16104015

Function and Biology Details

Reaction catalysed: 
Hydrolysis of -Gln(76)-|-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP).
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetanus toxin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 50.48 KDa
Source organism: Clostridium tetani
UniProt:
  • Canonical: P04958 (Residues: 875-1315; Coverage: 34%)
Gene names: CTC_p60, tetX
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TYR 1 x TYR
Ligand GLU 1 x GLU
Ligand TRP 1 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 66.757Å b: 79.183Å c: 90.265Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.274

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Citations

9 review citations

Botulinum and Tetanus Neurotoxins.
Dong et al. (2019)
8 more