7bst

Electron Microscopy
4.37Å resolution

EcoR124I-Ocr in the Intermediate State

Released:

Function and Biology Details

Reactions catalysed:
Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-137264 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit Chains: B, C
Molecule details ›
Chains: B, C
Length: 1038 amino acids
Theoretical weight: 120.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P10486 (Residues: 1-1038; Coverage: 100%)
Gene names: hsdR, hsr
Sequence domains:
Type I restriction enzyme EcoR124I/EcoR124II methylase subunit Chains: D, E
Molecule details ›
Chains: D, E
Length: 520 amino acids
Theoretical weight: 58.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P10484 (Residues: 1-520; Coverage: 100%)
Gene names: hsdM, hsm
Sequence domains:
Protein Ocr Chains: F, G
Molecule details ›
Chains: F, G
Length: 117 amino acids
Theoretical weight: 13.82 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P03775 (Residues: 1-117; Coverage: 100%)
Gene name: 0.3
Sequence domains: DNA mimic ocr
Type I restriction enzyme EcoR124I/EcoR124II specificity subunit Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 46.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P10485 (Residues: 1-404; Coverage: 100%)
Gene names: hsdS, hss
Sequence domains: Type I restriction modification DNA specificity domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.37Å
Relevant EMDB volumes: EMD-30166
Expression system: Escherichia coli