› 7b9w

X-ray diffraction
1.82Å resolution

Crystal structure of MurE from E.coli in complex with Z757284380

Released:
DOI: 10.2210/pdb7b9w/pdb
PDB entry 7b9w coloured by chain, front view.
PDB entry 7b9w coloured by chain, side view.
PDB entry 7b9w coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Koekemoer L, Steindel M, Fairhead M, Arrowsmith CH, Edwards AM, Bountra C, von Delft F, Krojer T, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 496 amino acids
Theoretical weight: 53.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22188 (Residues: 1-495; Coverage: 100%)
Gene names: JW0083, b0085, murE
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand T4H 1 x T4H
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P1
Unit cell:
a: 58.409Å b: 58.621Å c: 74.438Å
α: 97.11° β: 91.54° γ: 104.841°
R-values:
R R work R free
0.195 0.193 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links