› 6xgv

X-ray diffraction
2.11Å resolution

Crystal Structure of KRAS-G13D (GMPPNP-bound) in complex with RAS-binding domain (RBD) and cysteine-rich domain (CRD) of RAF1/CRAF

Released:
DOI: 10.2210/pdb6xgv/pdb
PDB entry 6xgv coloured by chain, front view.
PDB entry 6xgv coloured by chain, side view.
PDB entry 6xgv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
KRAS interaction with RAF1 RAS-binding domain and cysteine-rich domain provides insights into RAS-mediated RAF activation.
Tran TH, Chan AH, Young LC, Bindu L, Neale C, Messing S, Dharmaiah S, Taylor T, Denson JP, Esposito D, Nissley DV, Stephen AG, McCormick F, Simanshu DK
OpenAccess logo Nat Commun 12 1176 (2021)
PMID: 33608534

Function and Biology Details

Reactions catalysed: 
GTP + H(2)O = GDP + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133995 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase KRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-169; Coverage: 89%)
  • Best match: P01116-2 (Residues: 1-169)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
RAF proto-oncogene serine/threonine-protein kinase Chain: B
Molecule details ›
Chain: B
Length: 137 amino acids
Theoretical weight: 15.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04049 (Residues: 52-188; Coverage: 21%)
Gene names: RAF, RAF1
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
Ligand ZN 3 x ZN
Ligand GOL 2 x GOL
Ligand CL 1 x CL
1 modified residue:
Ligand CAF 1 x CAF

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P622
Unit cell:
a: 132.75Å b: 132.75Å c: 89.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.204
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Progress on Ras/MAPK Signaling Research and Targeting in Blood and Solid Cancers.
Dillon et al. (2021)
16 more