› 6pcz

X-ray diffraction
1.44Å resolution

Crystal structure of the bacterial cellulose synthase subunit G (BcsG) catalytic domain from Escherichia coli, selenomethionine variant

Released:
DOI: 10.2210/pdb6pcz/pdb
PDB entry 6pcz coloured by chain, front view.
PDB entry 6pcz coloured by chain, side view.
PDB entry 6pcz coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The Escherichia coli cellulose synthase subunit G (BcsG) is a Zn2+-dependent phosphoethanolamine transferase.
Anderson AC, Burnett AJN, Hiscock L, Maly KE, Weadge JT
J Biol Chem 295 6225-6235 (2020)
PMID: 32152228

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153461 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulose biosynthesis protein BcsG Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 45.91 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37659 (Residues: 164-559; Coverage: 71%)
Gene names: JW3506, b3538, bcsG, yhjU
Sequence domains: Cellulose biosynthesis protein BcsG

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 78.71Å b: 94.58Å c: 105.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Regulation of Biofilm Exopolysaccharide Production by Cyclic Di-Guanosine Monophosphate.
Poulin et al. (2021)
1 more

3 mentions without citation

Phosphoethanolamine Transferases as Drug Discovery Targets for Therapeutic Treatment of Multi-Drug Resistant Pathogenic Gram-Negative Bacteria.
Thai et al. (2023)
2 more