› 6p7c

X-ray diffraction
2.76Å resolution

D104A/S128A S. typhimurium siroheme synthase

Released:
DOI: 10.2210/pdb6p7c/pdb
PDB entry 6p7c coloured by chain, front view.
PDB entry 6p7c coloured by chain, side view.
PDB entry 6p7c coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Siroheme synthase orients substrates for dehydrogenase and chelatase activities in a common active site.
Pennington JM, Kemp M, McGarry L, Chen Y, Stroupe ME
Nat Commun 11 864 (2020)
PMID: 32054833

Function and Biology Details

Reactions catalysed: 
Precorrin-2 + NAD(+) = sirohydrochlorin + NADH
(1a) S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1
Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Siroheme synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 457 amino acids
Theoretical weight: 50.15 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P25924 (Residues: 1-457; Coverage: 100%)
Gene names: STM3477, cysG
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 59.94Å b: 99.913Å c: 147.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.184 0.285
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Diversity of the reaction mechanisms of SAM-dependent enzymes.
Sun et al. (2021)
3 other articles