› 6anj

X-ray diffraction
1.7Å resolution

Synaptotagmin-7, C2A domain

Released:
DOI: 10.2210/pdb6anj/pdb
PDB entry 6anj coloured by chain, front view.
PDB entry 6anj coloured by chain, side view.
PDB entry 6anj coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Exceptionally tight membrane-binding may explain the key role of the synaptotagmin-7 C2A domain in asynchronous neurotransmitter release.
Voleti R, Tomchick DR, Südhof TC, Rizo J
Proc Natl Acad Sci U S A 114 E8518-E8527 (2017)
PMID: 28923929

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178963 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Synaptotagmin-7 Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.87 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62747 (Residues: 134-262; Coverage: 32%)
Gene name: Syt7
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand TBU 3 x TBU
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 55.302Å b: 55.302Å c: 89.805Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.148 0.144 0.181
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Mechanism of neurotransmitter release coming into focus.
Rizo J. (2018)
7 more

2 mentions without citation

The high-affinity calcium sensor synaptotagmin-7 serves multiple roles in regulated exocytosis.
MacDougall et al. (2018)
1 more