› 5d9o

X-ray diffraction
1.55Å resolution

Crystal structure of PbGH5A, a glycoside hydrolase family 5 enzyme from Prevotella bryantii B14, E280A mutant in complex with cellotetraose

Released:
DOI: 10.2210/pdb5d9o/pdb
PDB entry 5d9o coloured by chain, front view.
PDB entry 5d9o coloured by chain, side view.
PDB entry 5d9o coloured by chain, top view.
Source organism: Prevotella bryantii
Primary publication:
Structure-Function Analysis of a Mixed-linkage β-Glucanase/Xyloglucanase from the Key Ruminal Bacteroidetes Prevotella bryantii B(1)4.
McGregor N, Morar M, Fenger TH, Stogios P, Lenfant N, Yin V, Xu X, Evdokimova E, Cui H, Henrissat B, Savchenko A, Brumer H
J Biol Chem 291 1175-97 (2016)
PMID: 26507654

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH26 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.33 KDa
Source organism: Prevotella bryantii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O06842 (Residues: 573-924; Coverage: 39%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 49.18Å b: 85.058Å c: 74.978Å
α: 90° β: 101.4° γ: 90°
R-values:
R R work R free
0.154 0.152 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Recent structural insights into the enzymology of the ubiquitous plant cell wall glycan xyloglucan.
Attia et al. (2016)
2 more

2 mentions without citation

Metadata Analysis Approaches for Understanding and Improving the Functional Involvement of Rumen Microbial Consortium in Digestion and Metabolism of Plant Biomass.
Kameshwar et al. (2019)
1 more