› 3sj5

X-ray diffraction
1.67Å resolution

I5F Mutant Structure of T. Tengcongensis H-NOX

Released:
DOI: 10.2210/pdb3sj5/pdb
PDB entry 3sj5 coloured by chain, front view.
PDB entry 3sj5 coloured by chain, side view.
PDB entry 3sj5 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Controlling conformational flexibility of an O₂-binding H-NOX domain.
Weinert EE, Phillips-Piro CM, Tran R, Mathies RA, Marletta MA
Biochemistry 50 6832-40 (2011)
PMID: 21721586

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting transducer domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 22.08 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RBX6 (Residues: 1-188; Coverage: 31%)
Gene names: TTE0680, Tar4
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 44.585Å b: 67.116Å c: 66.428Å
α: 90° β: 92.01° γ: 90°
R-values:
R R work R free
0.2 0.197 0.247
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Nitric oxide-sensing H-NOX proteins govern bacterial communal behavior.
Plate et al. (2013)
5 more

2 mentions without citation

Controlling conformational flexibility of an O₂-binding H-NOX domain.
Weinert et al. (2011)
1 more