› 3gp5

X-ray diffraction
2.25Å resolution

Crystal structure of phosphoglyceromutase from Burkholderia pseudomallei with 3-phosphoglyceric acid and vanadate

Released:
DOI: 10.2210/pdb3gp5/pdb
PDB entry 3gp5 coloured by chain, front view.
PDB entry 3gp5 coloured by chain, side view.
PDB entry 3gp5 coloured by chain, top view.
Source organism: Burkholderia pseudomallei
Primary publication:
An ensemble of structures of Burkholderia pseudomallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase.
Davies DR, Staker BL, Abendroth JA, Edwards TE, Hartley R, Leonard J, Kim H, Rychel AL, Hewitt SN, Myler PJ, Stewart LJ
OpenAccess logo Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1044-50 (2011)
PMID: 21904048

Function and Biology Details

Reaction catalysed: 
(1a) [enzyme]-L-histidine + 2,3-bisphospho-D-glycerate = [enzyme]-N(tau)-phospho-L-histidine + 2/3-phospho-D-glycerate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 28.96 KDa
Source organism: Burkholderia pseudomallei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JWH7 (Residues: 1-249; Coverage: 100%)
Gene names: BURPS1710b_0662, gpmA
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

4 bound ligands:
Ligand PG6 1 x PG6
Ligand VO4 2 x VO4
Ligand 3PG 2 x 3PG
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 44.749Å b: 48.983Å c: 62.96Å
α: 105.46° β: 91.15° γ: 107.42°
R-values:
R R work R free
0.174 0.171 0.234
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

Oxidative stress activates SIRT2 to deacetylate and stimulate phosphoglycerate mutase.
Xu et al. (2014)
8 more

2 mentions without citation

Structure and activity of the metal-independent fructose-1,6-bisphosphatase YK23 from Saccharomyces cerevisiae.
Kuznetsova et al. (2010)
1 more