› 3ga9

X-ray diffraction
2.3Å resolution

Crystal structure of Bacillus anthracis transpeptidase enzyme CapD, crystal form II

Released:
DOI: 10.2210/pdb3ga9/pdb
PDB entry 3ga9 coloured by chain, front view.
PDB entry 3ga9 coloured by chain, side view.
PDB entry 3ga9 coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Crystal structure of Bacillus anthracis transpeptidase enzyme CapD.
Wu R, Richter S, Zhang RG, Anderson VJ, Missiakas D, Joachimiak A
J Biol Chem 284 24406-14 (2009)
PMID: 19535342

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175962 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Capsule biosynthesis protein CapD large chain Chain: L
Molecule details ›
Chain: L
Length: 323 amino acids
Theoretical weight: 36.22 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51693 (Residues: 29-351; Coverage: 64%)
Gene names: BXB0063, GBAA_pXO2_0063, capD, dep, pXO2-55
Sequence domains: Gamma-glutamyltranspeptidase
Structure domains: Serum Albumin; Chain A, Domain 1
Capsule biosynthesis protein CapD small chain Chain: S
Molecule details ›
Chain: S
Length: 177 amino acids
Theoretical weight: 19.59 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51693 (Residues: 352-528; Coverage: 35%)
Gene names: BXB0063, GBAA_pXO2_0063, capD, dep, pXO2-55
Sequence domains: Gamma-glutamyltranspeptidase
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

1 bound ligand:
Ligand GLU 2 x GLU
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 47.704Å b: 72Å c: 128.853Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.252
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The surface of Bacillus anthracis.
Fouet A. (2009)
11 other articles

2 mentions without citation

Bacterial Gamma-Glutamyl Transpeptidase, an Emerging Biocatalyst: Insights Into Structure-Function Relationship and Its Biotechnological Applications.
Saini et al. (2021)
1 more