› 3ahs

X-ray diffraction
1.32Å resolution

Crystal Structure of Ustilago sphaerogena Ribonuclease U2B

Released:
DOI: 10.2210/pdb3ahs/pdb
PDB entry 3ahs coloured by chain, front view.
PDB entry 3ahs coloured by chain, side view.
PDB entry 3ahs coloured by chain, top view.
Source organism: Ustilago sphaerogena
Primary publication:
Structural changes induced by the deamidation and isomerization of asparagine revealed by the crystal structure of Ustilago sphaerogena ribonuclease U2B.
Noguchi S
Biopolymers 93 1003-10 (2010)
PMID: 20623666

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing adenosine = an (RNA fragment)-3'-adenosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease U2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.39 KDa
Source organism: Ustilago sphaerogena
UniProt:
  • Canonical: P00654 (Residues: 1-114; Coverage: 100%)
Gene name: RNU2
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

3 bound ligands:
Ligand PO4 3 x PO4
Ligand K 2 x K
Ligand GOL 2 x GOL
1 modified residue:
Ligand IAS 3 x IAS

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P3121
Unit cell:
a: 98.237Å b: 98.237Å c: 87.605Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.162 0.174

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Citations

3 review citations

Immunogenicity of Biotherapeutics: Causes and Association with Posttranslational Modifications.
Kuriakose et al. (2016)
2 more