› 2xfw

X-ray diffraction
1.65Å resolution

Structure of the E192N mutant of E. coli N-acetylneuraminic acid lyase in complex with pyruvate in crystal form III

Released:
DOI: 10.2210/pdb2xfw/pdb
PDB entry 2xfw coloured by chain, front view.
PDB entry 2xfw coloured by chain, side view.
PDB entry 2xfw coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Campeotto I, Murshudov GN, Bolt AH, Trinh CH, Phillips SEV, Nelson A, Pearson AR, Berry A

Function and Biology Details

Reaction catalysed: 
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylneuraminate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 304 amino acids
Theoretical weight: 33.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6L4 (Residues: 2-297; Coverage: 100%)
Gene names: JW3194, b3225, nanA, npl
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand PYR 6 x PYR
Ligand 1PE 4 x 1PE
1 modified residue:
Ligand KPI 4 x KPI

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 78.11Å b: 116.53Å c: 83.74Å
α: 90° β: 117.95° γ: 90°
R-values:
R R work R free
0.158 0.156 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Engineering specialized metabolic pathways--is there a room for enzyme improvements?
Bar-Even et al. (2013)
12 other articles

1 mention without citation

Pathological macromolecular crystallographic data affected by twinning, partial-disorder and exhibiting multiple lattices for testing of data processing and refinement tools.
Campeotto et al. (2018)