› 2p3q

X-ray diffraction
2.75Å resolution

Crystal Structure of Dengue Methyltransferase in Complex with GpppG and S-Adenosyl-L-homocysteine

Released:
DOI: 10.2210/pdb2p3q/pdb
PDB entry 2p3q coloured by chain, front view.
PDB entry 2p3q coloured by chain, side view.
PDB entry 2p3q coloured by chain, top view.
Source organism: Dengue virus 2
Primary publication:
Structural and functional analysis of methylation and 5'-RNA sequence requirements of short capped RNAs by the methyltransferase domain of dengue virus NS5.
Egloff MP, Decroly E, Malet H, Selisko B, Benarroch D, Ferron F, Canard B
J Mol Biol 372 723-36 (2007)
PMID: 17686489

Function and Biology Details

Reactions catalysed: 
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-173119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
type II methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 34.4 KDa
Source organism: Dengue virus 2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q20IJ2 (Residues: 2495-2787; Coverage: 9%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

3 bound ligands:
Ligand SO4 6 x SO4
Ligand GP3 1 x GP3
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3121
Unit cell:
a: 111.502Å b: 111.502Å c: 56.534Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.208 0.271
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Organelle-like membrane compartmentalization of positive-strand RNA virus replication factories.
den Boon et al. (2010)
36 more

6 mentions without citation

Structure and functionality in flavivirus NS-proteins: perspectives for drug design.
Bollati et al. (2010)
5 more