› 1b2o

X-ray diffraction
1.9Å resolution

CLOSTRIDIUM PASTEURIANUM RUBREDOXIN G10VG43A MUTANT

Released:
DOI: 10.2210/pdb1b2o/pdb
PDB entry 1b2o coloured by chain, front view.
PDB entry 1b2o coloured by chain, side view.
PDB entry 1b2o coloured by chain, top view.
Source organism: Clostridium pasteurianum
Primary publication:
Rubredoxin from Clostridium pasteurianum. Structures of G10A, G43A and G10VG43A mutant proteins. Mutation of conserved glycine 10 to valine causes the 9-10 peptide link to invert.
Maher MJ, Xiao Z, Wilce MC, Guss JM, Wedd AG
Acta Crystallogr D Biol Crystallogr 55 962-8 (1999)
PMID: 10216292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rubredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 54 amino acids
Theoretical weight: 6.11 KDa
Source organism: Clostridium pasteurianum
Expression system: Escherichia coli
UniProt:
  • Canonical: P00268 (Residues: 1-54; Coverage: 100%)
Sequence domains: Rubredoxin
Structure domains: Rubrerythrin, domain 2

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 61.85Å b: 61.85Å c: 80.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.237
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

A cluster exposed: structure of the Rieske ferredoxin from biphenyl dioxygenase and the redox properties of Rieske Fe-S proteins.
Colbert et al. (2000)
5 more