› 1a7j

X-ray diffraction
2.5Å resolution

PHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDES

Released:
DOI: 10.2210/pdb1a7j/pdb
PDB entry 1a7j coloured by chain, front view.
PDB entry 1a7j coloured by chain, side view.
PDB entry 1a7j coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
The crystal structure of phosphoribulokinase from Rhodobacter sphaeroides reveals a fold similar to that of adenylate kinase.
Harrison DH, Runquist JA, Holub A, Miziorko HM
Biochemistry 37 5074-85 (1998)
PMID: 9548738

Function and Biology Details

Reaction catalysed: 
ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-bisphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-146080 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribulokinase 1 Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.71 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12033 (Residues: 1-290; Coverage: 100%)
Gene name: prkA
Sequence domains: Phosphoribulokinase / Uridine kinase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P432
Unit cell:
a: 129.83Å b: 129.83Å c: 129.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.284
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

The ferredoxin/thioredoxin system of oxygenic photosynthesis.
Schürmann et al. (2008)
3 more

10 mentions without citation

Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate.
Meloni et al. (2023)
9 more