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"secondary-accession": "SRP047273",
"centre-name": "University of East Anglia",
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"study-abstract": "Methanol dehydrogenase is a key enzyme in methylotrophic bacteria, catalysing the oxidation of methanol to formaldehyde. In many methylotrophs, this is a well-characterised pyrroloquinoline quinone dependent enzyme consisting of two subunits, encoded by the genes mxaFI. A homolog of the large subunit MxaF is also present in most known methylotrophs, encoded by the gene xoxF. In several methylotrophs, this gene was found to be the only putative methanol dehydrogenase, but it is also present in several non-methylotrophs. This has led to a discussion over its potential role in methylotrophy and C1 cycling. This study aims to investigate the presence and diversity of the xoxF gene in the marine environment.",
"study-name": "Survey of xoxF diversity in different marine environments",
"data-origination": "HARVESTED"
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"last-update": "2019-11-07T16:30:32",
"secondary-accession": "SRP047230",
"centre-name": "University of East Anglia",
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"study-abstract": "Methanol is a biochemically active, volatile organic compound that plays an important role in tropospheric oxidant photochemistry. It has recently been suggested that the global ocean, where surface methanol concentrations are in the ~30-400 nM range, is a net sink for atmospheric methanol. In the marine environment, this (and other) one-carbon compound is used as an energy and carbon source by bacteria known as methylotrophs. So far, little is known about the identity of the active methanol oxidisers in marine habitats. In this study, Stable Isotope Probing using 13C labelled methanol was combined with 16S rRNA and functional gene amplicon sequencing as well as metagenome sequencing to identify methylotrophic bacteria that are responsible for methanol assimilation in samples obtained from the Western Channel Observatory station L4.",
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