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{
"data": {
"type": "samples",
"id": "SRS599226",
"attributes": {
"sample-metadata": [
{
"key": "geographic location (longitude)",
"value": "72.2",
"unit": null
},
{
"key": "geographic location (country and/or sea,region)",
"value": "Kerguelen area",
"unit": null
},
{
"key": "environment (biome)",
"value": "Marine",
"unit": null
},
{
"key": "geographic location (latitude)",
"value": "-48.5",
"unit": null
},
{
"key": "instrument model",
"value": "454 GS FLX Titanium",
"unit": null
},
{
"key": "last update date",
"value": "2015-06-23",
"unit": null
}
],
"longitude": 72.2,
"biosample": "SAMN02741409",
"latitude": -48.5,
"accession": "SRS599226",
"analysis-completed": "2019-04-05",
"collection-date": null,
"geo-loc-name": "Kerguelen area",
"sample-desc": "DNA extraction and pyrosequencing. Samples from the continuous culture experiment and from the natural sites were further analyzed together. A combined DNA-RNA extraction was performed on the 0.2 micrometer fractions after lysis and purification steps, as described in Landa et al., 2014. In total, 16 DNA samples were sent to the Molecular Research DNA laboratory (http://www.mrdnalab.com/) for 454 pyrosequencing. The sequencing was performed using an FLX-Titanium platform (Roche) as described in Wolcott et al. (2009). Universal bacterial primers 27F (5-AGRGTTTGATCMTGGCTCAG-3) and 530R (5-CCGCNGCNGCTGGCAC-3) were used, targeting hypervariable regions V1 to V3 of the 16S rRNA gene. Sequences from the continuous cultures and from stations R, A3.2 and F can be found on HUFUDAT01.sff, with the following barcodes: ACTGGTGT (Continuous culture, control, replicate 1), ACTGTCAG (Continuous culture, control, replicate 2), ACTGTCTC (Continuous culture, control, replicate 3), ACTGGACT (Continuous culture, +DOM treatment, replicate 1), ACTGGAGA (Continuous culture, +DOM treatment, replicate 2), ACTGGTCA (Continuous culture, +DOM treatment, replicate 3), ACTGTGAC (R), AGACAGAC (A3.2) and AGACCTCA (F). Sequences from stations A3.1, A3.3, A3.4, E1, E3, E4.E and E4.W can be found on HUFUDAT02.sff, with the following barcodes: ACCGCTAC (A3.1), ACCGGCTT (A3.3), ACCGTAGA (A3.4), AGACGTCT (E1), AGACTGAG (E3), ACAGTGAA (E4.E) and AGAGAGAG (E4.W).",
"environment-biome": "Marine",
"environment-feature": null,
"environment-material": null,
"sample-name": "KEOPS2 (bacterial diversity)",
"sample-alias": "KEOPS2 (bacterial diversity)",
"host-tax-id": null,
"species": null,
"last-update": "2019-04-05T21:05:38"
},
"relationships": {
"runs": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS599226/runs?format=api"
}
},
"studies": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS599226/studies?format=api"
},
"data": [
{
"type": "studies",
"id": "MGYS00004211",
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00004211?format=api"
}
}
]
},
"biome": {
"data": {
"type": "biomes",
"id": "root:Environmental:Aquatic:Marine"
},
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Aquatic:Marine?format=api"
}
}
},
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS599226?format=api"
}
}
}