Sample Instance

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{
    "data": {
        "type": "samples",
        "id": "SRS386986",
        "attributes": {
            "biosample": "SAMN01894147",
            "sample-metadata": [
                {
                    "key": "host taxid",
                    "value": "9606",
                    "unit": null
                },
                {
                    "key": "host scientific name",
                    "value": "Homo sapiens",
                    "unit": null
                }
            ],
            "latitude": null,
            "longitude": null,
            "accession": "SRS386986",
            "analysis-completed": null,
            "collection-date": null,
            "geo-loc-name": null,
            "sample-desc": "After the removal of supragingival plaque with sterile gauze, individual subgingival plaque samples were taken from the mesio-buccal aspect of four molar teeth in four quadrants (upper right, upper left, lower right and lower left) per subject using sterile periodontal curettes (Hu-Friedy, Chicago, IL). Each sample was placed in a separate sterile 1.5-ml tube containing 200 *l TE buffer (50 mM Tris-HCl, 1 mM EDTA; pH 7.6). Bacterial DNA extraction was performed using commercially available DNA purification kit (Epicentre MasterPure™, Madison, WI) according to manufacturer’s guidelines. First, the debris was separated by centrifugation at 4*C and supernatant was transferred to another microcentrifuge tube and pellet was discarded. The collected supernatant was mixed with 500 *l of isopropanol and centrifuged at 4*C for DNA isolation. Isopropanol solution was carefully removed without dislodging the DNA pellet. The DNA pellet was rinsed with 75% ethanol and residual ethanol was removed. The sample was resuspended in 25 *l of TE buffer and stored at −80*C until analysis.",
            "environment-biome": null,
            "environment-feature": null,
            "environment-material": null,
            "sample-name": "s1-case",
            "sample-alias": "s1-case",
            "host-tax-id": 9606,
            "species": "Homo sapiens",
            "last-update": "2020-11-17T18:44:37"
        },
        "relationships": {
            "biome": {
                "data": {
                    "type": "biomes",
                    "id": "root:Host-associated:Human:Digestive system:Oral:Subgingival plaque"
                },
                "links": {
                    "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Human:Digestive%20system:Oral:Subgingival%20plaque?format=api"
                }
            },
            "studies": {
                "links": {
                    "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS386986/studies?format=api"
                },
                "data": [
                    {
                        "type": "studies",
                        "id": "MGYS00005651",
                        "links": {
                            "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00005651?format=api"
                        }
                    },
                    {
                        "type": "studies",
                        "id": "MGYS00005655",
                        "links": {
                            "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00005655?format=api"
                        }
                    }
                ]
            },
            "runs": {
                "links": {
                    "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS386986/runs?format=api"
                }
            }
        },
        "links": {
            "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS386986?format=api"
        }
    }
}