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"accession": "MGYS00001227",
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"last-update": "2016-09-23T11:27:17",
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"centre-name": "PURDUE UNIVERSITY",
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"study-abstract": "Characterization of microbial communities has become useful in studying the ties between organismal health and the host microbiome. Pathogens currently threaten the existence of many amphibian species, and some studies have characterized the amphibian cutaneous microbiome. Hellbender salamanders (Cryptobranchus alleganiensis) provide an ideal system to explore the relationship between microbiome and disease due to differences in health between the two hellbender subspecies. The Ozark hellbender subspecies (C. a. bishopi) currently exhibits chronic wounds believed to be caused by bacterial infections, whereas the eastern hellbender (C. a. alleganiensis) does not. Through bacterial 16s rRNA amplicon sequencing, we detected more than 8,000 distinct operational taxonomic units in the cutaneous and environmental microbiome. We found differences in the bacterial communities between the two subspecies, while but there were no differences between the Ozark wound and healthy samples nor between respective subspecies environment samples. Several opportunistic pathogens were found to have an association with Ozark hellbenders. These findings suggest that wounds present only in the Ozark hellbenders may be due to a reduced immunocompetence relative to eastern hellbenders. Using next generation sequencing allowed for an in-depth exploration of the distribution and abundance of bacteria between two threatened amphibian subspecies.",
"study-name": "Hellbender cutaneous microbiomes",
"data-origination": "SUBMITTED"
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