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{
"data": {
"type": "samples",
"id": "ERS756543",
"attributes": {
"biosample": "SAMEA3461645",
"longitude": 8.434,
"sample-metadata": [
{
"key": "sample storage temperature",
"value": "-80",
"unit": "\\'||chr(38)||\\'deg;C"
},
{
"key": "temperature",
"value": "17.725",
"unit": "\\'||chr(38)||\\'deg;C"
},
{
"key": "investigation type",
"value": "mimarks-survey",
"unit": null
},
{
"key": "project name",
"value": "PRJEB9624",
"unit": null
},
{
"key": "collection date",
"value": "2012-07-04",
"unit": null
},
{
"key": "sample collection device or method",
"value": "syringe",
"unit": null
},
{
"key": "amount or size of sample collected",
"value": "0.002",
"unit": "L"
},
{
"key": "target gene",
"value": "16s rRNA",
"unit": null
},
{
"key": "target subfragment",
"value": "V1-V2",
"unit": null
},
{
"key": "pcr primers",
"value": "27f-338r,27F: TCAGAGTTTGATCMTGGCTCAG + 338r: CATGCTGCCTCCCGTAGGAGT",
"unit": null
},
{
"key": "multiplex identifiers",
"value": "f: TCTGTCAC; r: GCTTGGAT",
"unit": null
},
{
"key": "pcr conditions",
"value": "initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles",
"unit": null
},
{
"key": "sequencing method",
"value": "Sequencing by synthesis, Illumina paired-end",
"unit": null
},
{
"key": "chemical administration",
"value": "Mix of ampiciline, gentamicine, tetracycline and kanamycine, each 100 µg/l seawater each",
"unit": null
},
{
"key": "host common name",
"value": "Pacific oyster",
"unit": null
},
{
"key": "host taxid",
"value": "29159",
"unit": null
},
{
"key": "perturbation",
"value": "treated with mix of ampiciline, gentamicine, tetracycline and kanamycine (100 µg/l seawater each) between 2012-05-29 and 2012-06-01",
"unit": null
},
{
"key": "NCBI sample classification",
"value": "29159",
"unit": null
},
{
"key": "instrument model",
"value": "Illumina MiSeq",
"unit": null
},
{
"key": "ENA checklist",
"value": "GSC MIxS host associated (ERC000013)",
"unit": null
},
{
"key": "geographic location (region and locality)",
"value": "Schleswig Holstein, Sylt, List, Odewatt",
"unit": null
},
{
"key": "host body habitat",
"value": "hemolymph",
"unit": null
},
{
"key": "host body product",
"value": "NA",
"unit": null
},
{
"key": "host body site",
"value": "adductor muscle",
"unit": null
},
{
"key": "host life stage",
"value": "adult",
"unit": null
},
{
"key": "sample volume or weight for DNA extraction",
"value": "0.2",
"unit": "mL"
},
{
"key": "host-associated environmental package",
"value": "host-associated",
"unit": null
}
],
"latitude": 55.0297,
"accession": "ERS756543",
"analysis-completed": "2017-03-21",
"collection-date": null,
"geo-loc-name": "North Sea",
"sample-desc": "Pacific oyster (Crassostrea gigas) hemolymph microbiota; collected on Sylt, 2012-07-04; sample taken after field deployment; treated with mix of ampiciline, gentamicine, tetracycline and kanamycine (100 µg/l seawater each) between 2012-05-29 and 2012-06-01; oyster from Texel, translocated from Texel (NL) to Sylt (D)",
"environment-biome": "aquatic, marine, benthic, coastal",
"environment-feature": "organic",
"environment-material": "organic material, bodily fluid",
"sample-name": "Pacific oyster hemolymph microbiota from Sylt, translocated from Texel (NL)",
"sample-alias": "Cg_88_Hem_Sy_TxA_20120704",
"host-tax-id": 29159,
"species": "Crassostrea gigas",
"last-update": "2017-03-21T11:44:24"
},
"relationships": {
"biome": {
"data": {
"type": "biomes",
"id": "root:Host-associated:Mollusca:Digestive system"
},
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Host-associated:Mollusca:Digestive%20system?format=api"
}
},
"studies": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS756543/studies?format=api"
},
"data": [
{
"type": "studies",
"id": "MGYS00001607",
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00001607?format=api"
}
}
]
},
"runs": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS756543/runs?format=api"
}
}
},
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/ERS756543?format=api"
}
}
}