Sample Studies Relationship List

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            "type": "studies",
            "id": "MGYS00001716",
            "attributes": {
                "accession": "MGYS00001716",
                "bioproject": "PRJEB9483",
                "samples-count": 33,
                "is-private": false,
                "last-update": "2017-04-27T15:10:50",
                "secondary-accession": "ERP010592",
                "centre-name": "IGB BERLIN",
                "public-release-date": null,
                "study-abstract": "To evaluate if changes in salinity affecting diversity of particle-associated (PA) and free-living (FL) bacteria, we sampled both bacterial fractions in two seasons (summer and fall/winter) in surface waters of three selected stations of the Baltic Sea representing marine, mesohaline and oligohaline environments. All samples were PCR amplified with 30 cycles using the primer pair Bakt_341F (CCTACGGGNGGCWGCAG) and Bakt_805R (ACHVGGGTATCTAATCC). Primers were tailed with sample-specific 5 bp barcodes a 454-adptor region, spanning variable regions V3 and V4 of the 16S rRNA gene. PCR products were purified with Agencourt AMPure XP magnetic beads (Beckman Coulter GmbH, Krefeld, Germany), quantified with a Picogreen assay (LifeTechnologies, Carlsbad, USA), diluted and equally pooled. The bidirectional sequencing was performed on a 454 platform with Titanium Flex chemistry (Roche etc.).",
                "study-name": "Diversity of particle-attached bacteria in the Baltic Sea",
                "data-origination": "SUBMITTED"
            },
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                            "id": "root:Environmental:Aquatic:Marine",
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